In vivo two-photon Ca2+ imaging

During in vivo 2p imaging, the CA1 region of the hippocampus of an awake animal is examined. The experimental animal must solve various tasks in a virtual reality environment for reward. While the animal's head is fixed under the microscope, it can run freely on a linear treadmill belt. In the meantime, we measure the running speed, record its licks coming to the drinking port, and make a video of its face. Later, it helps us to monitor changes in the pupil and the usage of the whiskers.